The basal medium and especially the feed should contain a balanced set of essential nutrients in a ratio that meets the demand of the cells for cell proliferation and production of the pharmaceutical protein. A feed is added to the culture when a certain culture state is reached, such as when a certain cell density is reached, a certain culture time point is reached, or a certain nutrient concentration is reached. In a fed-batch culture, CHO cells are inoculated and start to grow in a basal medium. Chemically defined basal media and feeds are currently the standard for CHO cell fed-batch cultures. Selecting a suitable culture medium and feed platform is therefore pivotal for developing a fed-batch manufacturing process. These outputs are a function of viable cell density (VCD), specific productivity (q p), and culture longevity, which in turn are influenced by the medium and feed composition. For a fed-batch process volumetric productivity and product titer are important outputs. Fed-batch culture is currently the most common industrial process for CHO cell culture. The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent.Ĭhinese Hamster Ovary (CHO) cells are the main production host for recombinant therapeutic proteins (Wurm 2004). Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. Higher amino acid feeding did not always lead to higher mAb production. Two CHO cell clones derived from the same parental CHO BC® cell line and producing the same monoclonal antibody (BC-G, a low producing clone BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures.

Ivcd cell culture